Biomolecular AND Logic Gate Based on Immobilized Enzymes with Precise Spatial Separation Controlled by Scanning Electrochemical Microscopy.

Citation:

Gdor E, Katz E, Mandler D. Biomolecular AND Logic Gate Based on Immobilized Enzymes with Precise Spatial Separation Controlled by Scanning Electrochemical Microscopy. J. Phys. Chem. BJournal of Physical Chemistry B. 2013;117 (50) :16058 - 16065.

Date Published:

2013///

Abstract:

A surface-localized enzymic AND gate based on scanning electrochem. microscopy was designed and studied. The gate is composed of an insulating glass surface modified with the enzyme glucose oxidase (GOx) and another surface opposing it made of a microelectrode. The latter was modified with a second enzyme, invertase (INV). The distance sepg. the modified microelectrode and surface controlled the output of the AND gate produced upon the biocatalytic reaction of the confined enzymes. Specifically, as the GOx-modified glass substrate entered the diffusion layer of the microelectrode, it catalyzed the regeneration of an electron-transfer mediator, ferroceniummethanol, generated electrochem. at the tip by oxidizing glucose, also generated at the tip, by catalytic cleaving of sucrose by INV. To enhance the activity of the GOx, mutarotase was added to convert α- to β-glucose to be further consumed by GOx. Hence, an increase of the current at the microelectrode was obtained by approaching the glass surface only in the presence of all the components. This is the first micrometer-sized biomol. logic gate, of which the authors are aware, that is surface-confined and shows the promise held by the localization of biomol. information-processing species. [on SciFinder(R)]

Notes:

CAPLUS AN 2013:1815066(Journal; Online Computer File)