@article {7043, title = {Photosensitized NAD(P)H regeneration systems. Application in the reduction of butan-2-one, pyruvic, and acetoacetic acids and in the reductive amination of pyruvic and oxoglutaric acid to amino acid.}, journal = {J. Chem. Soc., Perkin Trans. 2Journal of the Chemical Society, Perkin Transactions 2: Physical Organic Chemistry (1972-1999)}, number = {6}, year = {1986}, note = {CAPLUS AN 1986:586857(Journal)}, month = {1986///}, pages = {805 - 11}, abstract = {NADH and NADPH were formed by a photosensitized enzyme-catalyzed process. NADPH was formed in the presence of ferredoxin NADP-reductase with Ru(bpy)32+ (bpy = 2,2{\textquoteright}-bipyridine) as photosensitizer, Me viologen as primary electron acceptor, and (NH4)3 EDTA or 2-mercaptoethanol. Zn(II) meso-tetramethylpyridiniumporphyrin was used as photosensitizer for the photoinduced prodn. of NADH with the same reaction components but with lipoamide dehydrogenase as the enzyme catalyst. The photoinduced NADH/NADPH regeneration systems were coupled to secondary enzyme-catalyzed processes, e.g. the redn. of butan-2-one to butan-2-ol, pyruvic acid to lactic acid, or acetoacetic acid to β-hydroxybytyric acid; coupling to the reductive amination of pyruvic acid to alanine and of α-oxoglutaric acid to glutamic acid was also possible. The products showed high optical purity and the enzymes and coenzymes showed high turnover nos. and stability. [on SciFinder(R)]}, keywords = {enzyme catalysis NADH NADPH regeneration, photosensitization regeneration system NADH NADPH}, isbn = {0300-9580}, author = {Mandler, Daniel and Willner, Itamar.} }