Label-free femtomolar cancer biomarker detection in human serum using graphene-coated surface plasmon resonance chips.

Citation:

He L, Pagneux Q, Larroulet I, Serrano AY, Pesquera A, Zurutuza A, Mandler D, Boukherroub R, Szunerits S. Label-free femtomolar cancer biomarker detection in human serum using graphene-coated surface plasmon resonance chips. Biosens. Bioelectron.Biosensors & Bioelectronics. 2016 :Ahead of Print.

Date Published:

2016///

Abstract:

Sensitive and selective detection of cancer biomarkers is vital for the successful diagnosis of early stage cancer and follow-up treatment. Surface Plasmon Resonance (SPR) in combination with different amplification strategies is one of the anal. approaches allowing the screening of protein biomarkers in serum. Here we describe the development of a point-of-care sensor for the detection of folic acid protein (FAP) using graphene-based SPR chips. The exceptional properties of CVD graphene were exploited to construct a highly sensitive and selective SPR chip for folate biomarker sensing in serum. The specific recognition of FAP is based on the interaction between folic acid receptors integrated through π-stacking on the graphene coated SPR chip and the FAP analyte in serum. A simple post-adsorption of human serum:bovine serum albumin (HS:BSA) mixts. onto the folic acid modified sensor resulted in a highly anti-fouling interface, while keeping the sensing capabilities for folate biomarkers. This sensor allowed femtomolar (fM) detection of FAP, a detection limit well adapted and promising for quant. clin. anal. [on SciFinder(R)]

Notes:

CAPLUS AN 2016:220193(Journal)