Patterning and Characterization of Surfaces with Organic and Biological Molecules by the Scanning Electrochemical Microscope.

Date Published:



A novel approach for micropatterning of surfaces with org. and biol. microstructures using the scanning electrochem. microscope (SECM) is described. The approach is based on the introduction of the spatial resoln. by local deposition of Au particles followed by monolayer formation and functionalization. Specifically, Au patterns were deposited locally on Si wafers with the SECM as a result of the controlled anodic dissoln. of a Au microelectrode. The Au patterns were further used as microsubstrates for assembling cystamine monolayers to which either fluoresceine isothiocyanate (FIT) or glucose oxidase (GOD) were covalently attached. Characterization of the org. monolayers, as well as the biol. activity of the enzyme patterns, was carried out by fluorescence microscopy and the SECM, resp. [on SciFinder(R)]


CAPLUS AN 2000:453009(Journal)