@article {2970,
	title = {Barrier-to-autointegration factor is required to segregate and enclose chromosomes within the nuclear envelope and assemble the nuclear lamina.},
	journal = {Proc Natl Acad Sci U S A},
	volume = {102},
	number = {9},
	year = {2005},
	month = {2005 Mar 1},
	pages = {3290-5},
	abstract = {Barrier-to-autointegration factor (BAF) binds dsDNA, LEM-domain proteins, and lamins. Caenorhabditis elegans BAF requires Ce-lamin and two LEM-domain proteins (Ce-emerin and Ce-MAN1) to localize during nuclear assembly. It was unknown whether Ce-lamin and LEM proteins, in turn, depend on Ce-BAF (mutually dependent structural roles). RNA interference-mediated down-regulation of Ce-BAF caused gross defects in chromosome segregation, chromatin decondensation, and mitotic progression as early as the two-cell stage, and embryos died at the approximately 100-cell stage. Nuclear pores reassembled, whereas Ce-lamin, Ce-emerin, and Ce-MAN1 bound chromatin but remained patchy and disorganized. The nuclear membranes formed but failed to enclose anaphase-bridged chromatin. Time-lapse imaging showed two phenotypes: anaphase-bridged chromatin that eventually resolved, and segregated chromatin that returned to the midzone. Thus, the assembly of BAF, lamins, and LEM-domain proteins is mutually dependent, and is required to capture segregated chromosomes within the nascent nuclear envelope. Embryos that escaped lethality by down-regulation of Ce-BAF grew into sterile adults with misplaced distal tip cells and gonads, further suggesting that mild postembryonic reductions in BAF disrupt tissue-specific functions.},
	keywords = {Amino Acid Sequence, Animals, Caenorhabditis elegans, Chromosomes, Fluorescent Antibody Technique, Indirect, Microscopy, Electron, Transmission, Molecular Sequence Data, RNA Interference, Sequence Homology, Amino Acid},
	issn = {0027-8424},
	doi = {10.1073/pnas.0408364102},
	author = {Margalit, Ayelet and Segura-Totten, Miriam and Gruenbaum, Yosef and Wilson, Katherine L}
}
