@article {27331,
	title = {Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine},
	journal = {Nature Communications},
	volume = {13},
	number = {1},
	year = {2022},
	note = {Export Date: 06 April 2024; Cited By: 15},
	month = {2022},
	abstract = {In nature as in biotechnology, light-oxygen-voltage photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several light-oxygen-voltage receptors invariably retained productive, if often attenuated, signaling responses. Structures of a light-oxygen-voltage paradigm at around 1 {\r A} resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient light-oxygen-voltage receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to light-oxygen-voltage receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins. {\textcopyright} 2022, The Author(s).},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130041158\&doi=10.1038\%2fs41467-022-30252-4\&partnerID=40\&md5=187f392dfa79e54afd6fa10e1e35f9ec},
	author = {Dietler, J. and Gelfert, R. and Kaiser, J. and Borin, V. and Renzl, C. and Pilsl, S. and Ranzani, A.T. and Garc{\'\i}a de Fuentes, A. and Gleichmann, T. and Diensthuber, R.P. and Weyand, M. and Mayer, G. and Schapiro, I. and M{\"o}glich, A.}
}
