@article {1666,
	title = {Action of streptococcal haemolysins and proteolytic enzymes on Ehrlich ascites tumour cells},
	journal = {British journal of experimental pathology},
	volume = {40},
	number = {5},
	year = {1959},
	pages = {417-423},
	abstract = {IT has been recently shown that various strains
of
Streptococcus
pyoqenes
group
A
possess
a
cell-bound
haemolysin
(CBH)
which
can
be
released
from
the
streptococcal
cells
into
the
surrounding
medium
by
some
surface
active
materials
(Tween
40,
Tween
80,
Triton)
by
crystalline
albumin,
but
not
by
sonlic
energy
(Ginsburg
and
Grossowicz,
1957
;
Ginsburg,
1958;
Ginsburg
and
Grossowicz,
1959).
This
haemolytic
factor
was
designated
as
streptolysin
"
D
"
(SLD)
D
signifying
detergent.
SLD
is
distinguished
from
streptolysin
0
(SLO)
streptolysin
S
(SLS)
(Todd,
1938)
and
from
the
intracellular
haemolysin
(IH)
described
by
Schwab
(Schwab,
1956)
on
the
bases
of
different
substrate
requirements
for
formation,
sensitivity
to
U.N.
irradiation
and
to
sonic
energy
(Ginsburg,
1958;
Ginsburg
and
Gros-
sowicz,
unpublished).
Besides
haemolysing
RBC
of
various
animal
species
both
cell-free
and
cell-
bounid
SLD,
are
also
capable
of
injuring
and
killing
various
mamimalian
cells
in
vitro
(Ehrlich
ascites
tumour
cells,
fibroblasts,
amnion
cells,
leucocytes)
(Gins-
buirg,
1958
;
Ginsburg
and
Grossowicz,
1959).
The
purpose
of
the
present
study
is
to
show
that
Ehrlich
ascites
tuilour
cells
damaged
by
different
streptococcal
haemolysins
may
be
disintegrated
bv
various
proteolytic
enzymes
which
by
themselves
are
not
lethal},
	author = {Isaac Ginsburg}
}
