@article {17398,
	title = {Characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria},
	journal = {Food \& Function},
	volume = {8},
	number = {5},
	year = {2017},
	month = {2017},
	pages = {1955-1965},
	abstract = {\&nbsp;An extract prepared from cranberry juice by dialysis known as nondialyzable material (NDM) has been shown previously to possess anti-adhesion activity toward microbial species including oral bacteria, uropathogenic\&nbsp;Escherichia coli\&nbsp;and\&nbsp;Helicobacter pylori. Bioassay-guided fractionation of cranberry NDM was therefore undertaken to identify the anti-adhesive constituents. An aqueous acetone-soluble fraction (NDMac) obtained from Sephadex LH-20 inhibited adhesion-linked activities by oral bacteria, including co-aggregation of oral bacteria\&nbsp;Fusobacterium nucleatum\&nbsp;with\&nbsp;Streptococcus sanguinis\&nbsp;or\&nbsp;Porphyromonas gingivalis, and biofilm formation by\&nbsp;Streptococcus mutans. Analysis of NDMac and subsequent subfractions by MALDI-TOF MS and\&nbsp;1H NMR revealed the presence of A-type proanthocyanidin oligomers (PACs) of 3{\textendash}6 degrees of polymerization composed of (epi)catechin units, with some (epi)gallocatechin and anthocyanin units also present, as well as quercetin derivatives. Subfractions containing putative xyloglucans in addition to the mixed polyphenols also inhibit biofilm formation by\&nbsp;S. mutans\&nbsp;(MIC = 125{\textendash}250 μg mL-1). These studies suggest that the anti-adhesion activities of cranberry NDM on oral bacteria may arise from a combination of mixed polyphenol and non-polyphenol constituents.\&nbsp;},
	url = {http://pubs.rsc.org/en/content/articlepdf/2017/fo/c7fo00109f},
	author = {Neto, C. C. and Penndorf, K.A. and Feldman, M. and Meron-Sudai, S. and Zakay-Rones, Z. and Steinberg, D. and Fridman, M. and Kashman, Y. and Ginsburg, I. and Ofek, I. and Weiss, E. I.}
}
