@article {2141,
	title = {Measurement of croton oil induced rabbit ear swelling and evaluation of anti-inflammatory agents with a standard low pressure caliper},
	journal = {Skin Research and Technology},
	volume = {2},
	number = {3},
	year = {1996},
	pages = {142-145},
	abstract = {Background/airns: Carbobenzoxy-phenylalanyl-methionine (CBZ-Phe-Met), a known inhibitor of the chemotactic peptide N- formyl-methionyl-leucyl-phenylalanine (fMLP)  in vitro,  has  not been evaluated as a topical anti-inflammatory agent in vivo.  In order to measure the effect of CBZ-phe-met, one needs a repeat- able,  quantitative,  easily  obtainable  standard  measurement  of the  edema  formation.  In  this  study,  a  caliper  designed  for measuring soft materials was  used to evaluate the edema,  in- duced by croton oil on rabbit ears, as well as the effects of CBZ- phe-met. Methods: The model used in this study was croton-induced in- flammation on rabbit ears. A caliper for measuring soft materials ( European standard  DIN 863 part 3, manufactured  by  TESA Ltd.,  Renens,  Switzerland) was  used to  evaluate  the  edema, which is part of the inflammatory effect . The action of CBZ-phe- met and two other anti-inflammatory agents; hydrocortisone and Na-ibuprofen, were compared. 
Results: CBZ-phe-met 1-5\% was found to reduce the  edema on rabbit ears induced by croton oil by 15 to 93\%. 5\% CBZ-phe- met was found to be as effective as 5\% Na-ibuprofen and 0.1\% hydrocortisone. Conclusion: The caliper for soft materials was found to be suit- able for measuring the edema induced by croton-oil, as well as the  reducing edema due to anti-inflammatory treatment. It was also found that CBZ-phe-met is a potent topical anti-inflamma- tory  agent  in  the  croton-oil-induced  inflammatory  model.  This may indicate a new approach in the treatment of inflammation. Key words: Soft material caliper - inflammation - N-formyl-me- thionyl-leucyl-phenylalanine (fMLP) - carbobenzoxy-phenylala- nyl-methionyl (CBZ-phe-met).},
	author = {V. Manny-Aframian and A. Shafran and A. Zlotogorski and Isaac Ginsburg and S. Dikstein}
}
