Flow Cytometry: A New Method To Investigate the Properties of Water-in-Oil-in-Water Emulsions.

Citation:

Hai M, Bernath K, Tawfik D, Magdassi S. Flow Cytometry: A New Method To Investigate the Properties of Water-in-Oil-in-Water Emulsions. LangmuirLangmuir. 2004;20 (6) :2081 - 2085.

Date Published:

2004///

Abstract:

A new and facile method is presented to evaluate w/o/w emulsions contg. fluorescent markers by flow cytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" with a fluorescent marker or "blank" without the need for complicated sample prepn. The yield of prepn. of the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by this new method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased with increasing concn. of FITC-BSA inside the internal phase, just like the release fraction of NaCl as marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior in w/o/w emulsions are in agreement with results reported by more complicated methods. [on SciFinder(R)]