Publications

2022
Moshe Armon, Francesco Marra, Yehouda Enzel, Dorita Rostkier‐Edelstein, Chaim I. Garfinkel, Ori Adam, Uri Dayan, and Efrat Morin. 2022. “Reduced Rainfall in Future Heavy Precipitation Events Related to Contracted Rain Area Despite Increased Rain Rate.” Earth's Future, 10, 1, Pp. 1–19. Abstract
Heavy precipitation events (HPEs) can lead to deadly and costly natural disasters and are critical to the hydrological budget in regions where rainfall variability is high and water resources depend on individual storms. Thus, reliable projections of such events in the future are needed. To provide high-resolution projections under the RCP8.5 scenario for HPEs at the end of the 21 st century, and to understand the changes in sub-hourly to daily rainfall patterns, weather research and forecasting (WRF) model simulations of 41 historic HPEs in the eastern Mediterranean are compared with "pseudo global warming" simulations of the same events. This paper presents the changes in rainfall patterns in future storms, decomposed into storms' mean conditional rain rate, duration, and area. A major decrease in rainfall accumulation (-30% averaged across events) is found throughout future HPEs. This decrease results from a substantial reduction of the rain area of storms (-40%) and occurs despite an increase in the mean conditional rain intensity (+15%). The duration of the HPEs decreases (-9%) in future simulations. Regionally maximal 10-min rain rates increase (+22%), whereas over most of the region, long-duration rain rates decrease. The consistency of results across events, driven by varying synoptic conditions, suggests that these changes have low sensitivity to the specific synoptic evolution during the events. Future HPEs in the eastern Mediterranean will therefore likely be drier and more spatiotemporally concentrated, with substantial implications on hydrological outcomes of storms. Plain Language Summary Heavy precipitation events are large storms that can recharge freshwater reservoirs, but can also lead to hazardous outcomes such as flash floods. Therefore, understanding the impacts of climate change on such storms is critical. Here, a weather model similar to those used in weather forecasts is used to simulate heavy precipitation events in the eastern Mediterranean. A large collection of storms is simulated in pairs: (1) historic storms, selected for their high impact, and (2) the same storms placed in a global warming scenario projected for the end of the 21 st century. Using these simulations we ask how present-day storms would look like were they to occur at the warmer end of the 21 st century. The future storms are found to produce much less rainfall compared to their historic counterparts. This decrease in rainfall is attributed mainly to the reduction in the area covered by storms' rainfall, and happens despite increasing rainfall intensities. These results suggest that the region will be drier in the future with larger dry areas during storms; however, over short durations, it would rain more intensely over contracted areas-increasing local hazards associated with heavy precipitation events.
R. Palombo, L. Barneschi, L. Pedraza-González, D. Padula, I. Schapiro, and M. Olivucci. 2022. “Retinal chromophore charge delocalization and confinement explain the extreme photophysics of Neorhodopsin.” Nature Communications, 13, 1. Publisher's Version Abstract
The understanding of how the rhodopsin sequence can be modified to exactly modulate the spectroscopic properties of its retinal chromophore, is a prerequisite for the rational design of more effective optogenetic tools. One key problem is that of establishing the rules to be satisfied for achieving highly fluorescent rhodopsins with a near infrared absorption. In the present paper we use multi-configurational quantum chemistry to construct a computer model of a recently discovered natural rhodopsin, Neorhodopsin, displaying exactly such properties. We show that the model, that successfully replicates the relevant experimental observables, unveils a geometrical and electronic structure of the chromophore featuring a highly diffuse charge distribution along its conjugated chain. The same model reveals that a charge confinement process occurring along the chromophore excited state isomerization coordinate, is the primary cause of the observed fluorescence enhancement. © 2022, The Author(s).
Shaul R. Shenhav. 2022. “Review of, Semantic Network Analysis, Elad Segev(ed.).” Misgarot Media (Hebrew). Publisher's Version
E. Bouton and C. S. C. Asterhan. 2022. “Revisiting revoice: An inductive method for capturing revoice in classroom discussions.” Proceedings of the 16th International Conference of the Learning Sciences - ICLS 2022. International Society of the Learning Sciences. Publisher's Version Abstract

Within the scholarly field of academically productive classroom dialogue, several elusive, yet rich and potent discourse moves have received special attention. However, their rarity and complexity also poses significant challenges to meeting interrater reliability thresholds, and they are often omitted from quantitative research efforts. We propose a different approach for coding that circumvents these issues, called DECCA. In this presentation, we showcase this methodology by focusing on teacher revoice. We demonstrate how it is possible to deconstruct this complex phenomenon into smaller and simpler elements. We then code each turn in the corpus for the existence or absence of each element (which we term Dialogue Elements, DEs). Adding a post-coding, pre-analysis stage allows us to extract turns which contain the specific combination of DEs relevant for revoice and distinguish it from similar teacher dialogue facilitation moves. Theoretical and practical implications are discussed.

A. Rozenberg, I. Kaczmarczyk, D. Matzov, J. Vierock, T. Nagata, M. Sugiura, K. Katayama, Y. Kawasaki, M. Konno, Y. Nagasaka, M. Aoyama, I. Das, E. Pahima, J. Church, S. Adam, V.A. Borin, A. Chazan, S. Augustin, J. Wietek, J. Dine, Y. Peleg, A. Kawanabe, Y. Fujiwara, O. Yizhar, M. Sheves, I. Schapiro, Y. Furutani, H. Kandori, K. Inoue, P. Hegemann, O. Béjà, and M. Shalev-Benami. 2022. “Rhodopsin-bestrophin fusion proteins from unicellular algae form gigantic pentameric ion channels.” Nature Structural and Molecular Biology, 29, 6, Pp. 592 - 603. Publisher's Version Abstract
Many organisms sense light using rhodopsins, photoreceptive proteins containing a retinal chromophore. Here we report the discovery, structure and biophysical characterization of bestrhodopsins, a microbial rhodopsin subfamily from marine unicellular algae, in which one rhodopsin domain of eight transmembrane helices or, more often, two such domains in tandem, are C-terminally fused to a bestrophin channel. Cryo-EM analysis of a rhodopsin-rhodopsin-bestrophin fusion revealed that it forms a pentameric megacomplex (~700 kDa) with five rhodopsin pseudodimers surrounding the channel in the center. Bestrhodopsins are metastable and undergo photoconversion between red- and green-absorbing or green- and UVA-absorbing forms in the different variants. The retinal chromophore, in a unique binding pocket, photoisomerizes from all-trans to 11-cis form. Heterologously expressed bestrhodopsin behaves as a light-modulated anion channel. © 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.
Sylvain Gigan, Ori Katz, Hilton B. de Aguiar, Esben Ravn Andresen, Alexandre Aubry, Jacopo Bertolotti, Emmanuel Bossy, Dorian Bouchet, Joshua Brake, Sophie Brasselet, Yaron Bromberg, Hui Cao, Thomas Chaigne, Zhongtao Cheng, Wonshik Choi, Tomáš Čižmár, Meng Cui, Vincent R Curtis, Hugo Defienne, Matthias Hofer, Ryoichi Horisaki, Roarke Horstmeyer, Na Ji, Aaron K. LaViolette, Jerome Mertz, Christophe Moser, Allard P. Mosk, Nicolas C. Pégard, Rafael Piestun, Sebastien Popoff, David B. Phillips, Demetri Psaltis, Babak Rahmani, Hervé Rigneault, Stefan Rotter, Lei Tian, Ivo M. Vellekoop, Laura Waller, Lihong Wang, Timothy Weber, Sheng Xiao, Chris Xu, Alexey Yamilov, Changhuei Yang, and Hasan Yılmaz. 2022. “Roadmap on Wavefront Shaping and deep imaging in complex media.” Journal of Physics: Photonics, 4, 4, Pp. 042501. Publisher's Version
Sylvain Gigan, Ori Katz, Hilton Barbosa de Aguiar, Esben Andresen, Alexandre Aubry, Jacopo Bertolotti, Emmanuel Bossy, Dorian Bouchet, Josh Brake, and Sophie Brasselet. 2022. “Roadmap on Wavefront Shaping and deep imaging in complex media.” Journal of Physics: Photonics. Publisher's Version
Gideon Rahat. 2022. “’Upheavel’: From Party Politics to Personal Politics.” In Democracy Now, edited by Dana Blander and Maya Gayer, Pp. 131-143. Tel Aviv: HaUniversita HaMeshuderet. Publisher's Version
Adva Shpatz Dayan, Małgorzata Wierzbowska, and Lioz. Etgar. 5/24/2022. “Ruddlesden–Popper 2D Chiral Perovskite-Based Solar Cells.” Small Struct, 2022, 2200051, Pp. 1-9.
small_structures_-_2022_-_shpatz_dayan_-_ruddlesden_popper_2d_chiral_perovskite-based_solar_cells.pdf
T. Ganor, N. Mor, and J.D. Huppert. 2022. “Rumination and Emotional Modulation of the Attentional Blink.” Cognitive Therapy and Research, 46, Pp. 95-103.
Stener Lie, Annalisa Bruno, Lydia Helena Wong, and lioz etgar. 2/22/2022. “Semitransparent Perovskite Solar Cells with > 13% Efficiency and 27% Transperancy Using Plasmonic Au Nanorods.” ACS Appl. Mater. Interfaces, 2022, 14, 9, Pp. 11339–11349.
acsami.1c22748.pdf
Sequential and concerted C-C and C-O bond dissociation in the Coulomb explosion of 2-propanol
Dror Bittner, Krishnendu Gope, Ester Livshits, Roi Baer, and Daniel Strasser. 2022. “Sequential and concerted C-C and C-O bond dissociation in the Coulomb explosion of 2-propanol.” Journal of Chemical Physics, 157, 07, Pp. 074309. Publisher's Version
M. Maestre-Reyna, C.-H. Yang, E. Nango, W.-C. Huang, E.P.G. Ngurah Putu, W.-J. Wu, P.-H. Wang, S. Franz-Badur, M. Saft, H.-J. Emmerich, H.-Y. Wu, C.-C. Lee, K.-F. Huang, Y.-K. Chang, J.-H. Liao, J.-H. Weng, W. Gad, C.-W. Chang, A.H. Pang, M. Sugahara, S. Owada, Y. Hosokawa, Y. Joti, A. Yamashita, R. Tanaka, T. Tanaka, F. Luo, K. Tono, K.-C. Hsu, S. Kiontke, I. Schapiro, R. Spadaccini, A. Royant, J. Yamamoto, S. Iwata, L.-O. Essen, Y. Bessho, and M.-D. Tsai. 2022. “Serial crystallography captures dynamic control of sequential electron and proton transfer events in a flavoenzyme.” Nature Chemistry, 14, 6, Pp. 677 - 685. Publisher's Version Abstract
Flavin coenzymes are universally found in biological redox reactions. DNA photolyases, with their flavin chromophore (FAD), utilize blue light for DNA repair and photoreduction. The latter process involves two single-electron transfers to FAD with an intermittent protonation step to prime the enzyme active for DNA repair. Here we use time-resolved serial femtosecond X-ray crystallography to describe how light-driven electron transfers trigger subsequent nanosecond-to-microsecond entanglement between FAD and its Asn/Arg-Asp redox sensor triad. We found that this key feature within the photolyase-cryptochrome family regulates FAD re-hybridization and protonation. After first electron transfer, the FAD•− isoalloxazine ring twists strongly when the arginine closes in to stabilize the negative charge. Subsequent breakage of the arginine–aspartate salt bridge allows proton transfer from arginine to FAD•−. Our molecular videos demonstrate how the protein environment of redox cofactors organizes multiple electron/proton transfer events in an ordered fashion, which could be applicable to other redox systems such as photosynthesis. [Figure not available: see fulltext.] © 2022, The Author(s), under exclusive licence to Springer Nature Limited.
Serial Focus Groups: A longitudinal design for studying interactive discourse in political communication research
Christian Baden, Olga Pasitselska, Tali Aharoni, and Keren Tenenboim-Weinblatt. 2022. “Serial Focus Groups: A longitudinal design for studying interactive discourse in political communication research.” In 72nd ICA Annual Conference.
Serial focus groups: A longitudinal design for studying interactive discourse
Christian Baden, Olga Pasitselska, Tali Aharoni, and Keren Tenenboim Weinblatt. 2022. “Serial focus groups: A longitudinal design for studying interactive discourse.” International Journal of Qualitative Methods. Publisher's Version Abstract

 

Focus group methods specialize in the analysis of interactive discourse, but are only rarely employed as a stand-alone method to study such phenomena, owing to inherent limitations concerning the comparability and generalizability of findings. In this paper, we argue that focus groups undergo three kinds of transformations, involving changes in participants’ cognitive states, social ties, and discursive behavior, which raise both analytic challenges and valuable opportunities for the study of shared meanings and interactive negotiation processes in society. Introducing Serial Focus Groups, we extend familiar focus group designs as a method for studying interactive discourse in a longitudinal perspective, capitalizing on the analytic potentials raised by these transformations. Reviewing the methodological literature and drawing upon two large-scale focus group studies of socially interactive sense-making, we argue that serial focus groups can help overcome some of the limitations of cross-sectional focus groups and offer valuable new opportunities for analysis and validation.

 

Sharing and social media: The decline of a keyword?

This article revisits claims made a decade ago about the importance of the word “sharing” in the context of social network sites (SNSs). Based on an analysis of the home pages of 61 SNSs between the years 2011 and 2020, the findings incontrovertibly show that “sharing” has lost its central place in the terminology employed by social media platforms in their self-presentation. Where in the mid-2000s SNSs relied heavily on a rhetoric of sharing to promote their services, by 2020, this rhetoric had been almost entirely dropped. The research reported here implies that social media platforms no longer feel a need or desire to be associated with these cultural beliefs. Given this, questions are raised as to whether “sharing” remains a keyword for social media.

J. Dietler, R. Gelfert, J. Kaiser, V. Borin, C. Renzl, S. Pilsl, A.T. Ranzani, A. García de Fuentes, T. Gleichmann, R.P. Diensthuber, M. Weyand, G. Mayer, I. Schapiro, and A. Möglich. 2022. “Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine.” Nature Communications, 13, 1. Publisher's Version Abstract
In nature as in biotechnology, light-oxygen-voltage photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several light-oxygen-voltage receptors invariably retained productive, if often attenuated, signaling responses. Structures of a light-oxygen-voltage paradigm at around 1 Å resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient light-oxygen-voltage receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to light-oxygen-voltage receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins. © 2022, The Author(s).
Linoy Dery, Nitzan Shauloff, Yury Turkulets, Ilan Shalish, Raz Jelinek, and Daniel Mandler. 2022. “Size-Selective Detection of Nanoparticles in Solution and Air by Imprinting.” ACS SENSORS, 7, 1, Pp. 296-303.
Kfir Sulimany, Offek Tziperman, Yaron Bromberg, and Omri Gat. 2022. “Soliton-pair dynamical transition in mode-locked lasers.” Optica, 9, 11, Pp. 1260–1267. Publisher's Version Abstract
The self-assembly of solitons into nonlinear superpositions of multiple solitons plays a key role in the complex dynamics of mode-locked lasers. These states are extensively studied in light of their potential technological applications and their resemblance to molecules that offer opportunities for studying molecular interactions. However, progress along these endeavors is still held back by the lack of effective means to manipulate multi-soliton waveforms. Here we show it is possible to control inter-soliton interactions in mode-locked fiber lasers using a single control knob, the laser gain. We experimentally demonstrate a 2-orders-of-magnitude reduction in the separation of bound soliton pairs by sweeping the pumping current of the laser. The sweep induces a dynamical transition between a phase-incoherent loosely bound state and a phase-locked tightly bound state. Using numerical simulations and a simplified analytical model, we find that the dynamical transition is governed by noise-mediated interactions, which can be switched between repulsion and attraction. The discovery of a single control parameter that sets the nature of the inter-soliton interaction points to possibilities for controlling multi-soliton states for optical communication systems and pump-probe spectroscopy.

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