Publications

This study contributes to growing scholarly interest in teacher-led, on-the-job learning communities and how collaborative inquiry into practice can be supported in such 
contexts. We particularly focus on the relative advantages and limitations of three teacher team activity types: video analysis, peer consultation and pedagogical planning. Fifty-four 
transcribed teacher meeting excerpts were analyzed using the CLIP coding scheme for teacher collaborative inquiry into practice, assessing aspects of inquiry-based reasoning, collaboration and focus on pedagogy (teacher actions, student thinking and disciplinary content). Quantitative comparisons showed that, overall, collaborative inquiry into practice was lowest during peer consultations, in part because teachers were often not positioned as agents of change in such conversations. Teachers tended to inquire into each other’s ideas more often during pedagogical planning. Surprisingly, teacher team video analysis activities were not characterized by higher measures of attention to student thinking, nor inquiry orientation. Practical and theoretical implications are discussed. 

Political polarization, seen as a key threat to contemporary democracy, has been tied to the rise of digital social media. However, how this process develops in the context of a social media environment characterized by multiple platforms—with differing norms, contents, and affordances—has not been sufficiently explored. In the present article, we propose a distinction between positional polarization, that is, people’s view on a political issue, and interpretative polarization, that is, how that political issue is contextualized and understood. We use this distinction to examine an issue of political controversy in Israel, examining how polarization develops over time, on three social media platforms—Facebook, Twitter, and WhatsApp. We find that contrasting positions are strongly connected to conflicting interpretations, both of which are clear from the start, with only minor overtime shifts. Moreover, while sharing broad similarities, the three platforms show a few distinctive polarization dynamics—both positional and interpretative—that can be connected to their varied socio-technical affordances. The study advances our theoretical understanding of polarization by examining how different social media platforms may shape distinct polarization dynamics over time, with different implications for democratic debate.

Phytochromes are biological photoswitches that interconvert between two parent states (Pr and Pfr). The transformation is initiated by photoisomerization of the tetrapyrrole chromophore, followed by a sequence of chromophore and protein structural changes. In the last step, a phytochrome-specific peptide segment (tongue) undergoes a secondary structure change, which in prokaryotic phytochromes is associated with the (de)activation of the output module. The focus of this work is the Pfr-to-Pr photoconversion of the bathy bacteriophytochrome Agp2 in which Pfr is the thermodynamically stable state. Using spectroscopic techniques, we studied the structural and functional consequences of substituting Arg211, Tyr165, His278, and Phe192 close to the biliverdin (BV) chromophore. In Pfr, substitutions of these residues do not affect the BV structure. The characteristic Pfr properties of bathy phytochromes, including the protonated propionic side chain of ring C (propC) of BV, are preserved. However, replacing Arg211 or Tyr165 blocks the photoconversion in the Meta-F state, prior to the secondary structure transition of the tongue and without deprotonation of propC. The Meta-F state of these variants displays low photochemical activity, but electronic excitation causes ultrafast alterations of the hydrogen bond network surrounding the chromophore. In all variants studied here, thermal back conversion from the photoproducts to Pfr is decelerated but substitution of His278 or Phe192 is not critical for the Pfr-to-Pr photoconversion. These variants do not impair deprotonation of propC or the α-helix/β-sheet transformation of the tongue during the Meta-F-to-Pr decay. Thus, we conclude that propC deprotonation is essential for restructuring of the tongue.Phytochromes are biological photoswitches that interconvert between two parent states (Pr and Pfr). The transformation is initiated by photoisomerization of the tetrapyrrole chromophore, followed by a sequence of chromophore and protein structural changes. In the last step, a phytochrome-specific peptide segment (tongue) undergoes a secondary structure change, which in prokaryotic phytochromes is associated with the (de)activation of the output module. The focus of this work is the Pfr-to-Pr photoconversion of the bathy bacteriophytochrome Agp2 in which Pfr is the thermodynamically stable state. Using spectroscopic techniques, we studied the structural and functional consequences of substituting Arg211, Tyr165, His278, and Phe192 close to the biliverdin (BV) chromophore. In Pfr, substitutions of these residues do not affect the BV structure. The characteristic Pfr properties of bathy phytochromes, including the protonated propionic side chain of ring C (propC) of BV, are preserved. However, replacing Arg211 or Tyr165 blocks the photoconversion in the Meta-F state, prior to the secondary structure transition of the tongue and without deprotonation of propC. The Meta-F state of these variants displays low photochemical activity, but electronic excitation causes ultrafast alterations of the hydrogen bond network surrounding the chromophore. In all variants studied here, thermal back conversion from the photoproducts to Pfr is decelerated but substitution of His278 or Phe192 is not critical for the Pfr-to-Pr photoconversion. These variants do not impair deprotonation of propC or the α-helix/β-sheet transformation of the tongue during the Meta-F-to-Pr decay. Thus, we conclude that propC deprotonation is essential for restructuring of the tongue.

book review

The circadian and endocrine systems influence many physiological processes in animals, but little is known on the ways they interact in insects. We tested the hypothesis that juvenile hormone (JH) influences circadian rhythms in the social bumble bee Bombus terrestris. JH is the major gonadotropin in this species coordinating processes such as vitellogenesis, oogenesis, wax production, and behaviors associated with reproduction. It is unknown however, whether it also influences circadian processes. We topically treated newly-emerged bees with the allatoxin Precocene-I (P-I) to reduce circulating JH titers and applied the natural JH (JH-III) for replacement therapy. We repeated this experiment in three trials, each with bees from different source colonies. Measurements of ovarian activity suggest that our JH manipulations were effective; bees treated with P-I had inactive ovaries, and this effect was fully recovered by subsequent JH treatment. We found that JH augments the strength of circadian rhythms and the pace of rhythm development in individually isolated newly emerged worker bees. JH manipulation did not affect the free-running circadian period, overall level of locomotor activity, sleep amount, or sleep structure. Given that acute manipulation at an early age produced relatively long-lasting effects, we propose that JH effects on circadian rhythms are mostly organizational, accelerating the development or integration of the circadian system.

Juvenile hormone (JH) is a key regulator of insect development and reproduction. Given that JH commonly affects adult insect fertility, it has been hypothesized to also regulate behaviors such as dominance and aggression that are associated with reproduction. We tested this hypothesis in the bumblebee Bombus terrestris for which JH has been shown to be the major gonadotropin. We used the allatoxin precocene-I (P-I) to reduce hemolymph JH titers and replacement therapy with JH-III to revert this effect. In small orphan groups of workers with similar body size but mixed treatment, P-I treated bees showed lower aggressiveness, oogenesis, and dominance rank compared with control and replacement therapy treated bees. In similar groups in which all bees were treated similarly, there was a clear dominance hierarchy, even in P-I and replacement therapy groups in which the bees showed similar levels of ovarian activation. In a similar experiment in which bees differed in body size, larger bees were more likely to be dominant despite their similar JH treatment and ovarian state. In the last experiment, we show that JH manipulation does not affect dominance rank in groups that had already established a stable dominance hierarchy. These findings solve previous ambiguities concerning whether or not JH affects dominance in bumblebees. JH positively affects dominance, but bees with similar levels of JH can nevertheless establish dominance hierarchies. Thus, multiple factors including JH, body size, and previous experience affect dominance and aggression in social bumblebees.

Gonadotropic hormones coordinate processes in diverse tissues regulating animal reproductive physiology and behavior. Juvenile hormone (JH) is the ancient and most common gonadotropin in insects, but not in advanced eusocial honey bees and some ants. To start probing the evolutionary basis of this change, we combined endocrine manipulations, transcriptomics, and behavioral analyses to study JH regulated processes in a bumble bee showing a relatively simple level of eusociality. We found that in worker fat body, more JH-regulated genes were up- rather than down-regulated, and enriched for metabolic and biosynthetic pathways. This transcriptomic pattern is consistent with earlier evidence that JH is the major gonadotropin in bumble bees. In the brain, more JH-regulated genes were down- rather than up-regulated and enriched for protein turnover pathways. Brain ribosomal protein gene expression shows a similar trend of downregulation in dominant workers, which naturally have high JH titers. In other species, similar downregulation of protein turnover is found in aging brains or under stress, associated with compromised long-term memory and health. These findings suggest a previously unknown gonadotropin-mediated tradeoff. Analysis of published data reveals no such downregulation of protein turnover pathways in the brain of honey bee workers, which exhibit more complex eusociality and in which JH is not a gonadotropin but rather regulates division of labor. These results suggest that the evolution of complex eusociality in honey bees was associated with modifications in hormonal signalling supporting extended and extremely high fertility while reducing the ancient costs of high gonadotropin titers to the brain.

The dynamics and the decay processes of inner-shell excited atoms are of great interest in physics, chemistry, biology, and technology. The highly excited state decays very quickly through different channels, both radiative and non-radiative. It is therefore a long-standing goal to study such dynamics directly in the time domain. Using few-cycle infrared laser pulses, we investigated the excitation and ionization of inner-shell electrons through laser-induced electron re-collision with the original parent ions and measured the dependence of the emitted x-ray spectra on the intensity and ellipticity of the driving laser. These directly re-colliding electrons can be used as the initiating pump step in pump/probe experiments for studying core-hole dynamics at their natural temporal scale. In our experiment we found that the dependence of the x-ray emission spectrum on the laser intensity and polarization state varies distinctly for the two kinds of atomic systems. Relying on our data and numerical simulations, we explain this behavior by the presence of different excitation mechanisms that are contributing in different ratios to the respective overall x-ray emission yields. Direct re-collision excitation competes with indirect collisions with neighboring atoms by electrons having &\#x201C;drifted away&\#x201D; from the original parent ion.

Generalized Kohn−Sham density functional theory is a popular computational tool for the ground state of extended systems, particularly within range-separated hybrid (RSH) functionals that capture the long-range electronic interaction. Unfortunately, the heavy computational cost of the nonlocal exchange operator in RSH-DFT usually confines the approach to systems with at most a few hundred electrons. A significant reduction in the computational cost is achieved by representing the density matrix with stochastic orbitals and a stochastic decomposition of the Coulomb convolution (J. Phys. Chem. A 2016, 120, 3071). Here, we extend the stochastic RSH approach to excited states within the framework of linear-response generalized Kohn−Sham time-dependent density functional theory (GKS-TDDFT) based on the plane-wave basis. As a validation of the stochastic GKS-TDDFT method, the excitation energies of small molecules N2 and CO are calculated and compared to the deterministic results. The computational efficiency of the stochastic method is demonstrated with a two-dimensional MoS2 sheet (∼1500 electrons), whose excitation energy, exciton charge density, and (excited state) geometric relaxation are determined in the absence and presence of a point defect.

The study of the human response to injury has been hampered by the inherent heterogeneity in the models and methods used. By studying a standard injury longitudinally, using individual patient‐level analysis, we endeavoured to better describe its dynamics. We analysed clinical variables, clinical laboratory and plasma cytokines from 20 patients at five time points. Clustering analysis showed two prototype patterns of cytokine behaviour: a concordant type, where cytokines behave the same way for all patients (notably IL‐0 and TNFα), and a variable type, where different patterns of expression are seen for different patients (notably IL‐8, IL‐6 and IL‐1RA). Analysis of the cytokines at the individual patient‐level showed a strong four‐way correlation between IL‐1RA, GCSF, MIP‐1β and MCP‐1. As it holds for most patients and not just on average, this suggests that they form a network which may play a central role in the response to gastro‐intestinal injuries in humans. In conclusion, the longitudinal analysis of cytokines in a standard model allowed the identification of their underlying patterns of expression. We propose that the two prototype patterns shown may reflect the mechanism that separates the common and individual aspects of the injury response.

We argue that housing affordability is as much about incomes as it is about house prices. Consequently, a comprehensive analysis of housing affordability should be conducted in which incomes and house prices are determined through the specification of labor, capital and product markets in addition to housing markets. A spatial econometric model for Israel is used to study the effects on the regional distribution of housing affordability of income generating shocks in labor and capital markets, as well as supply shocks in housing markets. Particular attention is paid to the effects on affordability of planning delays in tendering land for housing construction and the issue of building permits. Spatiotemporal impulse responses for housing affordability show that region-specific shocks, such as accelerated planning permission and the provision of regional investment grants, percolate across the economy as a whole. Implications for place-based regional policy are discussed.