@article { ISI:000365079800004,
	title = {Metabolomic analysis of the green microalga Chlamydomonas reinhardtii cultivated under day/night conditions},
	journal = {JOURNAL OF BIOTECHNOLOGY},
	volume = {215},
	year = {2015},
	month = {DEC 10},
	pages = {20-26},
	abstract = {Biomass composition of Chlamydomonas reinhardtii was studied during two consecutive cycles of 12 h light/12 h dark. As in our experimental conditions the two synchronized divisions were separated by 20 h, it was possible to show that accumulation of dry weight, proteins, chlorophyll and fatty acids mainly depends on cell division, whereas starch accumulation depends on a circadian rhythm as reported previously. Our metabolomics analyses also revealed that accumulation of five (Ser, Val, Leu, Ile and Thr) of the nine free amino acids detected displayed rhythmicity, depending on cell division while Glu was 20-50 times more abundant than the other ones probably because this free amino acid serves not only for protein synthesis but also for biosynthesis of nitrogen compounds. In addition, we performed a thermodynamic-motivated theoretical approach known as {\textquoteleft}surprisal analysis{\textquoteright}. The results from this analysis showed that cells were close to a steady state all along the 48 h of the experiment. In addition, calculation of free energy of cellular metabolites showed that the transition point, i.e. the state which immediately precedes cell division, corresponds to the most unstable stage of the cell cycle and that division is identified as the greatest drop in the free energy of metabolites. (C) 2015 Elsevier B.V. All rights reserved.},
	issn = {0168-1656},
	doi = {10.1016/j.jbiotec.2015.04.012},
	author = {Willamme, Remi and Alsafra, Zouheir and Arumugam, Rameshkumar and Eppe, Gauthier and Remacle, Francoise and LEVINE, RD and Remacle, Claire}
}
