Citation:
![Stirring Peptide Synthesis to a New Level of Efficiency Stirring Peptide Synthesis to a New Level of Efficiency](https://scholars.huji.ac.il/sites/default/files/styles/book_cover/public/mattanhurevich/files/op1c00304_0005_2.gif?m=1641838478&itok=IsprTUPy)
Abstract:
Accelerating solid-phase synthesis is crucial for accessing a large number of peptides in a short time. Since standard peptide synthesis is usually done under poor diffusion conditions with slow or no mixing of the solid support, acceleration of the process is achieved by applying a large excess of reagents. In this work, overhead stirring and heating were combined to provide accelerated solid-phase peptide synthesis without using an excess of reagent. A new setup that allows both heating and fast stirring was designed specifically for research laboratory-scale peptide synthesis. By increasing the diffusion of both reagents and beads in a narrow dimension reactor, solid-phase reactions were done in seconds and medium-size peptides were synthesized in minutes.