Li H, Mignolet B, Wachter G, Skruszewicz S, Zherebtsov S, Suessmann F, Kessel A, Trushin SA, Kling NG, Kuebel M, et al. Coherent Electronic Wave Packet Motion in C-60 Controlled by the Waveform and Polarization of Few-Cycle Laser Fields. PHYSICAL REVIEW LETTERS. 2015;114.
AbstractStrong laser fields can be used to trigger an ultrafast molecular response that involves electronic excitation and ionization dynamics. Here, we report on the experimental control of the spatial localization of the electronic excitation in the C-60 fullerene exerted by an intense few-cycle (4 fs) pulse at 720 nm. The control is achieved by tailoring the carrier-envelope phase and the polarization of the laser pulse. We find that the maxima and minima of the photoemission-asymmetry parameter along the laser-polarization axis are synchronized with the localization of the coherent electronic wave packet at around the time of ionization.
Orbach R, Lilienthal S, Klein M, LEVINE RD, Remacle F, Willner I.
Ternary DNA computing using 3 x 3 multiplication matrices. CHEMICAL SCIENCE. 2015;6 :1288-1292.
AbstractNon-Boolean computations implementing operations on multi-valued variables beyond base 2 allow enhanced computational complexity. We introduce DNA as a functional material for ternary computing, and in particular demonstrate the use of three-valued oligonucleotide inputs to construct a 3 x 3 multiplication table. The system consists of two three-valued inputs of -1; 0; + 1 and a fluorophore/quencher functional hairpin acting as computational and reporter module. The interaction of the computational hairpin module with the different values of the inputs yields a 3 x 3 multiplication matrix consisting of nine nanostructures that are read out by three distinct fluorescence intensities. By combining three different hairpin computational modules, each modified with a different fluorophore/quencher pair, and using different sets of inputs, the parallel operation of three multiplication tables is demonstrated.
Yan T-M, Fresch B, LEVINE RD, Remacle F.
Information processing in parallel through directionally resolved molecular polarization components in coherent multidimensional spectroscopy. JOURNAL OF CHEMICAL PHYSICS. 2015;143.
AbstractWe propose that information processing can be implemented by measuring the directional components of the macroscopic polarization of an ensemble of molecules subject to a sequence of laser pulses. We describe the logic operation theoretically and demonstrate it by simulations. The measurement of integrated stimulated emission in different phase matching spatial directions provides a logic decomposition of a function that is the discrete analog of an integral transform. The logic operation is reversible and all the possible outputs are computed in parallel for all sets of possible multivalued inputs. The number of logic variables of the function is the number of laser pulses used in sequence. The logic function that is computed depends on the chosen chromophoric molecular complex and on its interactions with the solvent and on the two time intervals between the three pulses and the pulse strengths and polarizations. The outputs are the homodyne detected values of the polarization components that are measured in the allowed phase matching macroscopic directions, k(l), k(l) = Sigma(i) l(i) k(i) where k(i) is the propagation direction of the ith pulse and \l(i)\ is a set of integers that encodes the multivalued inputs. Parallelism is inherently implemented because all the partial polarizations that define the outputs are processed simultaneously. The outputs, which are read directly on the macroscopic level, can be multivalued because the high dynamical range of partial polarization measurements by nonlinear coherent spectroscopy allows for fine binning of the signals. The outputs are uniquely related to the inputs so that the logic is reversible. (C) 2015 AIP Publishing LLC.
Willamme R, Alsafra Z, Arumugam R, Eppe G, Remacle F, LEVINE RD, Remacle C.
Metabolomic analysis of the green microalga Chlamydomonas reinhardtii cultivated under day/night conditions. JOURNAL OF BIOTECHNOLOGY. 2015;215 :20-26.
AbstractBiomass composition of Chlamydomonas reinhardtii was studied during two consecutive cycles of 12 h light/12 h dark. As in our experimental conditions the two synchronized divisions were separated by 20 h, it was possible to show that accumulation of dry weight, proteins, chlorophyll and fatty acids mainly depends on cell division, whereas starch accumulation depends on a circadian rhythm as reported previously. Our metabolomics analyses also revealed that accumulation of five (Ser, Val, Leu, Ile and Thr) of the nine free amino acids detected displayed rhythmicity, depending on cell division while Glu was 20-50 times more abundant than the other ones probably because this free amino acid serves not only for protein synthesis but also for biosynthesis of nitrogen compounds. In addition, we performed a thermodynamic-motivated theoretical approach known as `surprisal analysis'. The results from this analysis showed that cells were close to a steady state all along the 48 h of the experiment. In addition, calculation of free energy of cellular metabolites showed that the transition point, i.e. the state which immediately precedes cell division, corresponds to the most unstable stage of the cell cycle and that division is identified as the greatest drop in the free energy of metabolites. (C) 2015 Elsevier B.V. All rights reserved.
Fresch B, Cipolloni M, Yan T-M, Collini E, LEVINE RD, Remacle F.
Parallel and Multivalued Logic by the Two-Dimensional Photon-Echo Response of a Rhodamine-DNA Complex. JOURNAL OF PHYSICAL CHEMISTRY LETTERS. 2015;6 :1714-1718.
AbstractImplementing parallel and multivalued logic operations at the molecular scale has the potential to improve the miniaturization and efficiency of a new generation of nanoscale computing devices. Two-dimensional photon-echo spectroscopy is capable of resolving dynamical pathways on electronic and vibrational molecular states. We experimentally demonstrate the implementation of molecular decision trees, logic operations where all possible values of inputs are processed in parallel and the outputs are read simultaneously, by probing the laser induced dynamics of populations and coherences in a rhodamine dye mounted on a short DNA duplex. The inputs are provided by the bilinear interactions between the molecule and the laser pulses, and the output values are read from the two-dimensional molecular response at specific frequencies. Our results highlights how ultrafast dynamics between multiple molecular states induced by light-matter interactions can be used as an advantage for performing complex logic operations in parallel, operations that are faster than electrical switching.
Remacle F, LEVINE RD.
Statistical thermodynamics of transcription profiles in normal development and tumorigeneses in cohorts of patients. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 2015;44 :709-726.
AbstractExperimental biology is providing the distribution of numerous different biological molecules inside cells and in body fluids of patients. Statistical methods of analysis have very successfully examined these rather large databases. We seek to use a thermodynamic analysis to provide a physical understanding and quantitative characterization of human cancers and other pathologies within a molecule-centered approach. The key technical development is the introduction of a Lagrangian. By imposing constraints the minimal value of the Lagrangian defines a thermodynamically stable state of the cellular system. The minimization also allows using experimental data measured at a number of different conditions to evaluate the steady-state distribution of biomolecules such as messenger RNAs. Thereby the number of effectively accessible quantum states of biomolecules is determined from the experimentally measured expression levels. With the increased resolution provided by the minimization of the Lagrangian one can differentiate between normal and diseased patients and further between disease subtypes. Each such refinement corresponds to imposing an additional constraint of biological origin. The constraints are the unbalanced ongoing biological processes in the system. MicroRNA expression level data for control and diseased lung cancer patients are analyzed as an example.